Single-Molecule Sequencing
نویسندگان
چکیده
منابع مشابه
Advances in Single-molecule Nucleic Acid Sequencing
The ability to quickly and accurately obtain sequence information from single molecules of DNA and RNA has far-reaching implications for our understanding of biology. In the work presented here, we have made several advances in the area of single-molecule DNA and RNA sequencing. Specifically, in attempting to increase the read length of DNA polymerase, we have assayed several custom synthesized...
متن کاملSingle Molecule Fluorescence Microscopy and its Applications to Single Molecule Sequencing by Cyclic Synthesis
1.0. Introduction 2.0. Background 2.1. Single Molecule Detection 2.2. Total Internal Reflection 2.3. FRET Theory 3.0. DNA Sequencing by Cyclic synthesis 3.1. Motivation 3.2. Surface Treatment 3.3. Polymerase Kinetics 3.4. Sequencing Strategies 3.4.1. Cyclic Synthesis using FRET 3.4.2. Real Time Imaging 3.4.3. Non FRET Imaging 3.4.4. Cleavable Linkers 3.4.5. Cleavable Terminators 3.4.6. Multi-Co...
متن کاملRead Mapping Algorithms for Single Molecule Sequencing Data
Single Molecule Sequencing technologies such as the Heliscope simplify the preparation of DNA for sequencing, while sampling millions of reads in a day. Simultaneously, the technology suffers from a significantly higher error rate, ameliorated by the ability to sample multiple reads from the same location. In this paper we develop novel rapid alignment algorithms for two-pass Single Molecule Se...
متن کاملContinuous base identification for single-molecule nanopore DNA sequencing.
A single-molecule method for sequencing DNA that does not require fluorescent labelling could reduce costs and increase sequencing speeds. An exonuclease enzyme might be used to cleave individual nucleotide molecules from the DNA, and when coupled to an appropriate detection system, these nucleotides could be identified in the correct order. Here, we show that a protein nanopore with a covalent...
متن کاملRefining noninvasive prenatal diagnosis with single-molecule next-generation sequencing.
The gradual elimination of risky procedures used to sample fetal material for prenatal diagnosis has been an important objective in medicine. It is often stated that more fetuses are lost due to such invasive procedures (amniocentesis and chorionic villus sampling) than are identified as carrying a chromosomal abnormality. Noninvasive prenatal diagnosis (NIPD) has developed substantially since ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Molecular Diagnostics
سال: 2020
ISSN: 1525-1578
DOI: 10.1016/j.jmoldx.2019.10.001